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Single-shot 3D optical microscopy that can capture high-resolution information over a large volume has broad applications in biology. Existing 3D imaging methods using point-spread-function (PSF) engineering often have limited depth of field (DOF) or require custom and often complex design of phase masks. We propose a new, to the best of our knowledge, PSF approach that is easy to implement and offers a large DOF. The PSF appears to be axially V-shaped, engineered by replacing the conventional tube lens with a pair of axicon lenses behind the objective lens of a wide-field microscope. The 3D information can be reconstructed from a single-shot image using a deep neural network. Simulations in a 10× magnification wide-field microscope show the V-shaped PSF offers excellent 3D resolution (<2.5 µm lateral and ∼15 µm axial) over a ∼350 µm DOF at a 550 nm wavelength. Compared to other popular PSFs designed for 3D imaging, the V-shaped PSF is simple to deploy and provides high 3D reconstruction quality over an extended DOF. 

Abstract We present the in-lab and on-sky performance for the upgraded 90 GHz focal plane of the Cosmology Large Angular Scale Surveyor, which had four of its seven detector wafers updated during the austral winter of 2022. The update aimed to improve the transition-edge-sensor (TES) stability and bias range and to realize the high optical efficiency of the sensor design. Modifications included revised circuit terminations, electrical contact between the TES superconductor and the normal metal providing the bulk of the bolometer heat capacity, and additional filtering on the TES bias lines. The upgrade was successful: 94% of detectors are stable down to 15% of the normal resistance, providing a wide overlapping range of bias voltages for all TESs on a wafer. The median telescope efficiency improved from $0.42_{-0.22}^{+0.15}$to $0.60_{-0.32}^{+0.10}$(68% quantiles). For the four upgraded wafers alone, median telescope efficiency increased to $0.65_{-0.06}^{+0.06}$. Given our efficiency estimate for the receiver optics, this telescope efficiency implies a detector efficiency exceeding 0.90. The overall noise-equivalent temperature of the 90 GHz focal plane improved from $19\mu \mathrm{K}\sqrt{\mathrm{s}}$to $9.7\mu \mathrm{K}\sqrt{\mathrm{s}}$. 

We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity at cellular resolution. Our microscope uses an adaptive sampling scheme with line illumination. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the sample, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies) and samples a large area of them in a single measurement. Such a scheme significantly increases the imaging speed and reduces the overall laser power on the brain tissue. Using this approach, we performed high-speed imaging of the neuronal activity in mouse cortexin vivo. Our method provides a sampling strategy in laser-scanning two-photon microscopy and will be powerful for high-throughput imaging of neural activity. 

We demonstrate a high-speed two-photon fluorescence microscope using line illumination with an adaptive sampling scheme. The illumination pattern is modulated by a digital micro-mirror device so only the regions of interest are illuminated and sampled. 

The Cosmology Large Angular Scale Surveyor (CLASS) is a telescope array observing the Cosmic Microwave Background (CMB) at frequency bands centered near 40, 90, 150, and 220 GHz. CLASS measures the CMB polarization on the largest angular scales to constrain the inflationary tensor-to-scalar ratio and the optical depth due to reionization. To achieve the long time-scale stability necessary for this measurement from the ground, CLASS utilizes a front-end, variable-delay polarization modulator on each telescope. Here we report on the improvements in stability afforded by front-end modulation using data across all four CLASS frequencies. Across one month of modulated linear polarization data in 2021, CLASS achieved median knee frequencies of 9.1, 29.1, 20.4, and 36.4 mHz for the 40, 90, 150, and 220 GHz observing bands. The knee frequencies are approximately an order of magnitude lower than achieved via CLASS pair-differencing orthogonal detector pairs without modulation. 

The Cosmology Large Angular Scale Surveyor (CLASS) telescope array surveys 75% of the sky from the Atacama desert in Chile at frequency bands centered near 40, 90, 150, and 220 GHz. CLASS measures the largest-angular scale (θ ≳ 1 ° ) CMB polarization with the aim of constraining the tensor-to-scalar ratio, r, measuring the optical depth to reionization, τ , to near the cosmic variance limit, and more. The CLASS Q-band (40 GHz), W-band (90 GHz), and dichroic high frequency (150/220 GHz) telescopes have been observing since June 2016, May 2018, and September 2019, respectively. On-sky optical characterization of the 40 GHz instrument has been published. Here, we present preliminary on-sky measurements of the beams at 90, 150, and 220 GHz, and pointing stability of the 90 and 150/220 GHz telescopes. The average 90, 150, and 220 GHz beams measured from dedicated observations of Jupiter have full width at half maximum (FWHM) of 0.615±0.019° , 0.378±0.005° , and 0.266 ± 0.008° , respectively. Telescope pointing variations are within a few % of the beam FWHM. 

Abstract The current and future cosmic microwave background (CMB) experiments fielding kilopixel arrays of transition-edge sensor (TES) bolometers require accurate and robust gain calibration methods. We simplify and refactor the standard TES model to directly relate the detector responsivity calibration and optical time constant to the measured TES current I and the applied bias current I b . The calibration method developed for the Cosmology Large Angular Scale Surveyor (CLASS) TES bolometer arrays relies on current versus voltage ( I – V ) measurements acquired daily prior to CMB observations. By binning Q -band (40 GHz) I – V measurements by optical loading, we find that the gain calibration median standard error within a bin is 0.3%. We test the accuracy of this I – V bin detector calibration method by using the Moon as a photometric standard. The ratio of measured Moon amplitudes between the detector pairs sharing the same feedhorn indicates a TES calibration error of 0.5%. We also find that, for the CLASS Q -band TES array, calibrating the response of individual detectors based solely on the applied TES bias current accurately corrects TES gain variations across time but introduces a bias in the TES calibration from data counts to power units. Since the TES current bias value is set and recorded before every observation, this calibration method can always be applied to the raw TES data and is not subject to I – V data quality or processing errors. 

Abstract The Cosmology Large Angular Scale Surveyor (CLASS) is a four-telescope array observing the largest angular scales (2≲ ℓ ≲ 200) of the cosmic microwave background (CMB) polarization. These scales encode information about reionization and inflation during the early universe. The instrument stability necessary to observe these angular scales from the ground is achieved through the use of a variable-delay polarization modulator as the first optical element in each of the CLASS telescopes. Here, we develop a demodulation scheme used to extract the polarization timestreams from the CLASS data and apply this method to selected data from the first 2 yr of observations by the 40 GHz CLASS telescope. These timestreams are used to measure the 1/ f noise and temperature-to-polarization ( T → P ) leakage present in the CLASS data. We find a median knee frequency for the pair-differenced demodulated linear polarization of 15.12 mHz and a T → P leakage of <3.8 × 10 −4 (95% confidence) across the focal plane. We examine the sources of 1/ f noise present in the data and find the component of 1/ f due to atmospheric precipitable water vapor (PWV) has an amplitude of 203 ± 12 μ K RJ s for 1 mm of PWV when evaluated at 10 mHz; accounting for ∼17% of the 1/ f noise in the central pixels of the focal plane. The low levels of T → P leakage and 1/ f noise achieved through the use of a front-end polarization modulator are requirements for observing of the largest angular scales of the CMB polarization by the CLASS telescopes.